Sujeet Kumar Curriculum vitae

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  • CURRICULUM VITAE

    SUJEET KUMAR, PhD

    CONTACT INFORMATION:

    Postdoctoral Researcher,

    Dr. Natividad Ruiz lab,

    Ohio State University,

    Columbus, Ohio, USA 43210.

    Email: kumar.673@osu.edu, sujeetkumar.lsu@gmail.com

    Contact No: +1-225-571-9361

    EDUCATIONAL QUALIFICATION

    2016-Current Postdoctoral Researcher, The Ohio state University, Columbus, Ohio,

    USA

    2011-2016 PhD (Biological Sciences), Louisiana State University (LSU), Baton

    Rouge, Louisiana, USA.

    2007-2011 B. Tech (Biotechnology), Vellore Institute of Technology (VIT) University,

    Vellore, India.

    AWARDS, HONOURS AND SCHOLARSHIPS

    May 2016 LSU Graduate Deans summer research assistantship from Graduate

    school, LSU, Baton Rouge, LA.

    April 2016 Simon Chang / Ezzat Younathan Outstanding Biochemistry Teaching

    Assistant Award from the Department of Biological Science, LSU, Baton

    Rouge, LA.

    September 2015 Charles S. McCleskey award for best oral presentation at American

    Society for Microbiology, South Central Branch meeting, Hattiesburg, MS.

    June 2015 Bruch Award from the Department of Biological Science, LSU, Baton

    Rouge, LA, to attend Gordon Research Conference on Membrane

    transport and function in Lewiston, ME.

    mailto:kumar.673@osu.edu

  • April 2015 C.R Komma Memorial outstanding International graduate student

    award from the Department of Biological Science, LSU, Baton Rouge,

    LA.

    May 2014 McDaniel Award from the Department of Biological Science, LSU, Baton

    Rouge, LA, to attend American Society of Microbiology (ASM)

    Conference, Boston, MA.

    November 2013 Oral presentation Award in BioGrad Symposium 2013, Biological

    Sciences, LSU, Baton Rouge, LA.

    2011-present BioGrads Travel award for various research meetings from the

    Department of Biological Science, LSU, Baton Rouge, LA.

    May 2010 537th All India Rank in Graduate Aptitude Test in Engineering (GATE).

    October 2009 Awarded first in Bioquest Quiz held at VIT by SABEST student chapter.

    March 2009 Awarded first in Bio Savant Event in Riviera held at VIT.

    November 2008 Awarded third in Skip It event organized by the Youth Red Cross Society

    at VIT.

    July 2007 Merit scholarship for securing 722nd rank out of 88,000 students for VIT

    Engineering entrance examination.

    RESEARCH EXPERIENCE Doctoral Research: Department of Biological Sciences, Louisiana State University, Baton Rouge. (2011-Present).

    (Research Advisor: Dr. William T. Doerrler.)

    Investigation of the physiological functions of integral inner membrane proteins YqjA and YghB belonging to the ancient and highly conserved DedA protein family in Escherichia coli, drug resistance and alkaline pH tolerance.

    Extraction, solubilization and purification of YqjA and YghB for biochemical studies.

    Undergraduate Research: Department of Life Sciences, Jawaharlal Nehru University, New Delhi, India. (December 2010 -

    July 2011) (Research Advisor: Dr. Sneha Sudha Komath.)

    http://www.google.co.in/url?sa=t&source=web&cd=1&ved=0CCEQFjAA&url=http%3A%2F%2Fwww.gate.iitb.ac.in%2Fgate2011%2F&ei=zgfRTMX-FIimcILhxNAL&usg=AFQjCNF6TgHUN7ezp1JcuYKVHRSTpsoKtg

  • Mutational analysis of N-acetyl-D-glucosaminylphosphatidylinositol de-N-acetylase

    (PIG-L) from Entamoeba histolytica.

    School of Bio Sciences and Technology, VIT University, Vellore, India. (December 2008 -

    December 2010) (Research Advisor: Dr. V.Suneetha)

    Bioremediation of Keratinic waste using Actinomycetes.

    RESEARCH PUBLICATIONS

    1. Kumar Sujeet & Doerrler WT (2015) E. coli YqjA, a member of the conserved DedA/Tvp38

    membrane protein family, is a putative osmosensing transporter required for growth at

    alkaline pH J Bacteriol Apr 27. pii: JB.00175-15.

    2. Kumar Sujeet & Doerrler WT (2014) Members of the Conserved DedA Family Are Likely

    Membrane Transporters and Are Required for Drug Resistance in Escherichia coli.

    Antimicrob Agents Chemother 58:923-930. doi: 10.1128/AAC.02238-13.

    3. Doerrler WT, Sikdar R, Kumar Sujeet & Boughner LA (2013) New functions for the ancient

    DedA membrane protein family. J Bacteriol 195:3-11. doi: 10.1128/JB.01006-12.

    4. Kumar Sujeet, Bradley Cersten, Mukashaya Patience & Doerrler WT. Identification of

    essential arginine residues of E. coli DedA/Tvp38 family membrane proteins YqjA and

    YghB. (In press).

    5. Kumar Sujeet & Doerrler WT. Expression of yqjA, a member of the conserved DedA

    membrane protein family, of Escherichia coli, is regulated by Sodium/Potassium ions and

    alkaline pH. (In preparation 2016).

    CONFERENCES/SEMINAR/POSTER PRESENTATIONS

    April 2016 YqjA: Represents a New Family of Putative Transporter Essential for

    Alkaline pH Tolerance in Escherichia Coli.

    Sujeet Kumar and William T. Doerrler. ASBMB general meeting in San

    Diego, CA.

    March 2016 Role of members of conserved DedA/Tvp38 membrane protein

    family in E. coli drug resistance and alkaline pH tolerance

    Sujeet Kumar. Icahn School of Medicine at Mount Sinai, New York

    (Hosted by: Dr. Terry Krulwich).

  • November 2015 Role of DedA/Tvp38 membrane protein family in E. coli alkaline pH

    tolerance.

    Sujeet Kumar and William T. Doerrler. Oral presentation at BioGrads Symposium, Biological sciences, LSU, Baton Rouge, LA.

    September 2015 Members of conserved DedA/Tvp38 membrane protein family are required for growth of E. coli at alkaline pH.

    Sujeet Kumar and William T. Doerrler. Oral presentation at ASM south central branch meeting, Hattiesburg, MS.

    July 2015 Site Directed mutational analysis of DedA/Tvp38 membrane protein family members in E. coli.

    Patience Mukashyaka, Sujeet Kumar, Cersten Bradley and William T. Doerrler. Summer Undergraduate Research Forum, LSU, Baton Rouge, LA.

    June 2015 Members of the Conserved DedA/Tvp38 Membrane Protein Family are required for Escherichia Coli alkaline pH Homeostasis.

    Sujeet Kumar and William T. Doerrler. Mechanisms of Membrane Transport, Gordon Research Conference, Lewiston, ME.

    May 2015 Expression of yqjA, a member of the conserved DedA membrane protein family, of Escherichia coli, is regulated by Sodium/Potassium ions and alkaline pH.

    Sujeet Kumar and William T. Doerrler. ASM general meeting in New Orleans, LA.

    November 2014 A role for YqjA, a member of the conserved DedA membrane protein family, in alkaline pH homeostasis in E. coli.

    Sujeet Kumar and William T. Doerrler. Oral presentation at BioGrads Symposium, Biological sciences, LSU, Baton Rouge, LA.

    May 2014 A role for YqjA, a member of the conserved DedA membrane protein family, in alkaline pH homeostasis in E. coli.

    Sujeet Kumar and William T. Doerrler. ASM General Meeting in Boston, MA.

    November 2013 Members of the conserved DedA family are membrane transporters

    required for drug resistance in E. coli.

    Sujeet Kumar and William T. Doerrler. ASM Branch Meeting, New Orleans, LA.

    October 2013 Members of the conserved DedA family are membrane transporters

    required for drug resistance in E. coli.

  • Sujeet Kumar and William T. Doerrler. Oral presentation at BioGrads

    Symposium, Biological sciences, LSU, Baton Rouge, LA.

    TEACHING EXPERIENCE

    1. Graduate Teaching assistant, BIOL 4385 Undergraduate Biochemistry Lab, Louisiana

    State University (Fall 2011- present)

    Course co-ordinator: Dr. Johnna Roose and Dr. Erin Hawkins (Retd).

    General concepts and techniques of Molecular Biology and Biochemistry including gene cloning, Protein purification (Different chromatography techniques, SDS-PAGE, Western Blot), Enzyme Kinetics and Protein Structure exercises (Pymol, Crystallization) are discussed in BIOL 4385 Biochemistry Lab.

    Teaching and mentoring students on a weekly basis through lecture and demonstration in Lab.

    2. Graduate Teaching assistant, BIOL 1208 Freshman Introductory Biology Lab,

    Louisiana State University (Fall 2013)

    Course co-ordinator: Dr. E. William Wischusen

    General concepts in Biology, enzyme reaction, respiration, cellular metabolism, and Recombinant DNA technology are discussed in BIOL 1208.

    Teaching and mentoring students on a weekly basis through lecture and demonstration in Lab.

    TECHNIQUES AND SKILLS

    A. Experimental DNA/RNA: Cloning, Sequencing, qPCR, RT-PCR, Transformation, and Transduction, construction of cDNA/genomic DNA library, Chemical mutagen or Transposon mediated mutagenesis, Site-directed mutagenesis. Protein: 1D/2D SDS-PAGE, Western blotting, Protein expression and purification, preparation of membranes (inner and outer membrane fractions of gram-negative bacteria), Gel Filtration, Protein-protein interactions (IP, Co-IP, two-hybrid assays), Enzyme assays, Protein-DNA interaction (EMSA and DNAse footprinting). Lipids: Phospholipid extraction, 1D/2D Separation of lipid species (TLC), Quantification (Autoradiography).

  • Microscopy: Confocal microscopy, Differential Interference Contrast microscopy, Fluorescent Microscopy.

    B. Computational Bioinformatics: Proficient in basic Bioinformatics tools such as BLAST, Clustal W, Pymol.

    OUTREACH AND COMMUNITY Fall 2012-present Training of Undergraduate students in Dr. Doerrler Lab. Spring 2015-present Webmaster for Biograds, Biological Sciences, LSU. August 2015 Graduate Mentor for BIOS (Biology Intensive Orientation for

    Students) at LSU.

    Spring 2014-2015 General secretary for Indian Student Organization, LSU.

    August 2013 LINK program to assist new enrolled student at LSU.

    Summer 2010 Active participation in National Service Scheme organized by VIT.

    REFERENCES

    1. Dr. Natividad Ruiz,

    Department of Microbiology,

    The Ohio State University

    484 West 12th Avenue, Columbus, Ohio 43210

    Phone: (614) 292-3426; E-mail: ruiz.82@osu.edu

    2. Dr. William T. Doerrler

    Department of Biological Sciences

    Louisiana State University

    508 Life Sciences Building, Baton Rouge, LA 70803

    Phone: 225-578-7904, E-mail: wdoerr@lsu.edu

    3. Dr. Anne Grove

    Department of Biological Sciences

    Louisiana State University

    534 Choppin Hall, Baton Rouge, LA 70803

    Phone: (225) 578-5148, E-mail: agrove@lsu.edu

    http://sites01.lsu.edu/wp/bios/http://sites01.lsu.edu/wp/bios/mailto:ruiz.82@osu.eduhttp://www.biology.lsu.edu/index.htmhttp://www.lsu.edu/mailto:wdoerr@biochem.duke.eduhttp://www.biology.lsu.edu/index.htmhttp://www.lsu.edu/mailto:agrove@lsu.edu