Local Organising BRIDGING THE GAP BETWEEN SURGERY ?· BRIDGING THE GAP BETWEEN SURGERY AND BIOLOGY:…

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    16-Feb-2019

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<p> Local Organising Committee </p> <p> Alessia Di Giancamillo Laura Mangiavini </p> <p> Laura de Girolamo Antongiulio Marmotti </p> <p> Celeste Scotti </p> <p> BRIDGING THE GAP </p> <p> BETWEEN SURGERY AND BIOLOGY: </p> <p> HANDS-ON CARTILAGE </p> <p> LABORATORY COURSE </p> <p> Honorary President : Matteo Denti, Italy </p> <p> Program Chair: Giuseppe M. Peretti, Italy </p> <p> Helder Pereira, Portugal </p> <p> Congress Chairs: Giuseppe M. Peretti, Italy </p> <p> EVENT CO-ORGANIZED BY ESSKA </p> <p> CARTILAGE COMMITTEE AND </p> <p> RESEARCH COMMITTEE </p> <p> April 21 - 22 2016 DIBIT </p> <p> Fondazione Centro San Raffaele del Monte Tabor </p> <p> Honorary President </p> <p>Matteo Denti </p> <p>Program Chair Giuseppe M. Peretti </p> <p> Congress Chairs </p> <p> Giuseppe M. Peretti Helder Pereira </p> <p>Per migliorare la pratica clinica ci serve pi ricerca traslazionale! Lo scopo di questo corso quello di permettere ai chirurghi ortopedici di prendersi una pausa dalla pratica clinica per studiare pi approfonditamente la riparazione e rigenerazione cartilaginea. Il programma sar focalizzato sui diversi aspetti della ricerca in campo cartilagineo, dal prelievo di tessuto alla coltura cellulare e alle pi basilari tecniche di biologia molecolare e di istologia. Durante il corso, i partecipanti proveranno una vera esperienza di laboratorio conducendo loro direttamente diversi esperimenti; inoltre, i partecipanti acquisiranno una base teorica sulla riparazione cartilaginea fondamentale sia per intraprendere un eventuale cammino di ricerca di base nel campo della rigenerazione articolare, sia nel comprendere pi a fondo i principi che stanno alla base delle moderne metodiche di ingegneria dei tessuti, che al giorno doggi sono utilizzati quotidianamente nella pratica clinica. Questo corso rappresenta quindi unopportunit unica per ricercatori e chirurghi che desiderano avere un approccio diretto alla ricerca in campo cartilagineo. </p> <p>For a better clinical practice, we need more translational research! The aim of this course is to allow the orthopedic surgeons to take a short break from patients practice in order to go deeper inside cartilage repair and regeneration. The program will be focused on the different aspects of cartilage research, from tissue harvesting to cell culture and basic techniques of molecular biology and histology. The attendees will experience a real laboratory practice by directly conducting several lab experiments. Additionally, the attendees will acquire a theoretical background on cartilage repair, fundamental both for the research career in joint regeneration and for a greater comprehension of tissue engineering basic principles, which are nowadays routinely applied in clinical practice. Thus, this course represents a unique opportunity for scientists and surgeons, who desire a more direct approach on cartilage research. </p> <p> Laura de Girolamo (Milan, Italy) </p> <p>Matteo Denti (Milan, Italy) </p> <p>Alessia Di Giancamillo (Milan, Italy) </p> <p>Neil Fisher (Milan, Italy) </p> <p>Laura Mangiavini (Milan, Italy) </p> <p>AntonGiulio Marmotti (Turin, Italy) </p> <p>Silvia Mattia (Turin, Italy) </p> <p>Carlotta Perucca Orfei (Milan, Italy) </p> <p>Helder Pereira (Portugal) </p> <p>Giuseppe M. Peretti (Milan, Italy) </p> <p>Celeste Scotti (Milan, Italy) </p> <p>Irene Tessaro (Milan, Italy) </p> <p>Marco Vigan (Milan, Italy) </p> <p> FACULTY</p> <p> TUTORS </p> <p>Registration Introduction and presentation of the course </p> <p>THEORY </p> <p> Articular Cartilage morphology, biochemistry and biomechanics. Growth plate cartilage </p> <p> The articular environment Stem cells (MSC, ASC and mesenchymal cells </p> <p>from umbilical cord) Growth factors and Platelet Rich Plasma (PRP) Gene therapy Culture of chondrocytes: from monolayer to </p> <p> tridimensional (3D) culture </p> <p>Coffee break </p> <p> Molecular biology for gene expression analysis: RNA extraction, purification, quantification and measure of RNA integrity </p> <p> RT PCR PCR; semi-quantitative PCR and real time PCR Fluorocitometric analysis: Fluorescence </p> <p> Activated Cell Sorter (FACS) Analysis of protein expression and cell </p> <p>proliferation of chondrocytes both in monolayer and 3D cultures </p> <p> Use of different cell markers to determine cell phenotype and proliferation </p> <p> Immunohistochemistry an immunofluorescence of mesenchymal stem cells (differentiated versus undifferentiated) </p> <p> Immunohistochemistry and immunofluorescence of umbilical cord mesenchymal stem cells </p> <p>Lunch break </p> <p> First day (April 21st 2016) </p> <p> 8.30-9 am 9-9:30 am 9:30 am-1 pm </p> <p> 1 - 2 pm </p> <p>THEORY </p> <p> Engineered cartilage: animal models. Transgenic mouse models </p> <p> Biological materials for engineered cartilage Introduction to lab activity </p> <p>PRACTICE </p> <p> Articular explants from pig knee, shoulders and elbows </p> <p> Preparation of articular explants Preparation of the sterile field Collection of articular cartilage Preparation of culture medium, PBS with </p> <p>antibiotics/antimycotic and collagenase solution Basics on sterility in the use of the laminar flow </p> <p>cabinet Enzymatic digestion of articular cartilage </p> <p> First day (April 21st 2016) </p> <p> 2-2:40 pm </p> <p> 2.45 - 6 pm </p> <p> 1 - 2 pm </p> <p> PRACTICE </p> <p> Collection of chondrocytes Evaluation of cell number and viability In vitro cell culture: cell seeding and feeding; </p> <p>passaging, cell counts and viability 3D culture system: use of fibrin glue and </p> <p>collagen scaffold </p> <p>Lunch break </p> <p>THEORY </p> <p> Histology evaluation score, problems in the biopsy, preservation and storage </p> <p> Histology and staining on tissue sections: 1. Hematoxylin and Eosin (H&amp;E); different Hematoxylins 2. Safranin-O 3. Alcian Blue 4. Von Kossa 5. Massons Trichrome 6. TRAP 7. Immunohistochemistry and immunofluorescence 8. In situ hybridization </p> <p>PRACTICE </p> <p> H&amp;E Safranin-O staining and sample analysis under </p> <p>microscope Biochemistry assays: quantification of DNA and </p> <p>GAGs in 3D cultures versus native cartilage </p> <p>End of the Course </p> <p> Second day (April 22nd 2016) </p> <p> 9am - 1 pm 1 2 pm </p> <p> 2 -3 pm </p> <p> 3 - 5 pm </p> <p>DIBIT Fondazione Centro San Raffaele del Monte Tabor San Raffaele Congress Centre Via Olgettina, 58 - 20132 Milano </p> <p> Registration </p> <p> 480,00 (taxes included) </p> <p>Registrations are limited to maximum 20 </p> <p>attendees and will be accepted in the order in </p> <p>which they are received. Applications will have </p> <p>to the administrative office along with a copy of </p> <p>the money tranfer receipt. In case of cancellation, </p> <p>there will be no refund. </p> <p>Course fee includes: </p> <p> Attending to the entire theorical and </p> <p>practical sessions </p> <p> Lunch during the I and II course days </p> <p> Abstract book </p> <p> Keep International Via Giuseppe Vigoni, 11 - 20122 Milano Tel. +39 0254122579 Fax +39 0254124871 E.mail: info@keepinternational.net </p> <p>www.keywordeuropa.com </p> <p>GENERAL INFORMATION </p> <p>Venue </p> <p>Registration </p> <p>Organising </p> <p>Secretariat </p> <p> 1 - 2 pm </p>

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