Ascentis Express Peptide ES-C18 カラムでのペプチ Express Peptide ES-C18カラムでのペプチドマッピング はじめに ペプチドマッピングはタンパク質の主たる構造への 変化を評価するための確立した技術です。ペプチド マッピングは、タンパク質配列の変化やアミノ酸の化 学修飾を ...

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  • Ascentis Express Peptide ES-C18

    UV

    UV

    TFA

    TFA

    pH

    TFA

    UV

    UV

    TFA

    0.1%

    1ESI

    TFA

    1LC-MS

    TFA

    TFA

    MSFigure 1

    Hillel Brandes

    3

  • Supelco

    Fused-Core Ascentis Express

    Peptide ES-C18Fused-Core

    Table

    Ascentis Express Peptide ES-C18

    (Pc)tg

    wave

    Figure 2Table 2Figure 2Ascentis

    Express Peptide ES-C18C18

    Ascentis Express Peptide ES-C18

    Table 2

    Ascentis Express Peptide ES-C18

    0.1%LC-MS

    0.1%

    0.1%TFA2

    Figure 3

    TFA

    TFA

    McCalley2

    Table 3

    0.1%7%

    TFA98%

    TFA

    McCalley

    pHpH3.5

    7

    Figure 4

    pH3.5

    pH3

    pH3.5

    4

  • Ascentis Express Peptide ES-C18

    * all concentrations, shown as percentages, are v/v.

    ReferenceReferenceReferenceReference

    1. Apffel, A. et. al. 1995. Enhanced sensitivity for peptide mapping with

    electrospray liquid chromatography-mass spectrometry in the presence

    of signal suppression due to trifluoroacetic acid-containing mobile

    phases. J. Chrom A 712: 177-190.

    2. McCalley, D.V. 2004. Effect of buffer on peak shape of peptides in

    reversed-phase high performance liquid chromatography. J. Chrom A

    1038: 77-84.

    2012 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA, SAFC, SIGMA-ALDRICH, ALDRICH, and SUPELCO are trademarks of Sigma-Aldrich Co. LLC, registered in the US and other countries.

    FLUKA is a trademark of Sigma-Aldrich GmbH, registered in the US and other countries. SAFC brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the

    product(s) for their particular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at sigma-aldrich.com. Ascentis is a registered trademark of

    Sigma-Aldrich and Sigma-Aldrich Biotechnology, LP. Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

    140-0002 2-2-24 4

    TEL03-5796-7440 FAX03-5796-7355

    E-mailsialjpsp@sial.com

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    http://www.sigma-aldrich.com/japan

    2012111

    Websigma-aldrich.com/japan

    2012.11

    Ascentis Express Peptide ES-C18

    20,000 Da

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